BIOSYNTHESIS OF IRON OXIDE NANOPARTICLES USING CENTRAL COMPOSITE DESIGN OF RESPONSE SURFACE METHODOLOGY
Central Composite Design (CCD) of Response Surface Methodology has been applied in optimizing the
size of iron oxide nanoparticles (FeONPs) synthesised by studying the effects of three (3) important
elements which are reaction time, reaction temperature and extract volume. The quadratic model was
selected and fitness of the model was studied using sequential model of sum of squares and model of
summary statistics. The effects and interaction between the elements studied on the size of synthesised
FeONPs were investigated using analysis of variance (ANOVA), 2D contour plots and 3D surface plot.
ANOVA showed that the time of reaction has the greatest effect on the size of FeONPs synthesised and
the 3D surface plots revealed that to obtain the smallest size of FeONPs, the reaction time and the
reaction temperature has to be set at 15 mins & 25 oC respectively.